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| Registros recuperados: 233 | |
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| SAWAZAKI,HAIKO ENOK; NAGAI,IROSHI; SODEK,LADASLAV. |
| Estudou-se a variabilidade genética em couve (Brassica oleracea L. var. acephala D.C.) tipo manteiga por intermédio do polimorfismo enzimático em gel de poliacrilamida e do polimorfismo de DNA, denominado RAPD (Random Amplified Polymorphic DNA), com base na amplificação de segmentos de DNA ao acaso. Avaliaram-se quinze clones de couve-manteiga do Banco Ativo de Germoplasma do Instituto Agronômico (IAC), utilizando-se extratos de folhas para análise de isoenzimas e marcador RAPD com os "primers" dos kits A e B da Operon Technologies. Entre as isoenzimas estudadas, as mais polimórficas foram as fosfoglucomutase (PGM), peroxidase (PRX) e esterase (EST), tendo o sistema PGM realizado a melhor caracterização. Verificou-se a ocorrência de variabilidade genética... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Couve; Marcadores genéticos; Isoenzimas; RAPD. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051997000100002 |
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| Martinez,Sueli S.; Carvalho,Alfredo O. R. de; Vieira,Luiz G.; Nunes,Liliane M.; Bianchini,Anésio. |
| This work was carried out in order to identify Bemisia tabaci (Genn.) biotypes present in the state of Paraná and to determine their geographical distribution and host plants. About 50 adults were collected in several host crops, weeds and ornamental plants in North, Northwest, Northeast, West and Central areas of the state, from January to May, 1998 and 1999. The species were identified by means of RAPD-PCR, using the primer Operon H-16. Whitefly populations were detected mainly from February on, in both years, seldom achieving more than one adult per leaf. The insect was found in only 66% of the sampled areas. Bean golden mosaic was almost never observed during the period. Both biotype A and biotype B of B. tabaci were found in the State of Paraná, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Silverleaf whitefly; Sweetpotato whitefly; RAPD; DNA variability. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000300023 |
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| Riveros B.,Fernando; Muñoz,Gastón; González,Lucas; Rojas P.,Leonardo; Alvarez A.,Mario; Hinrichsen R.,Patricio. |
| Se evaluó la diversidad de especies de Fusarium asociadas con la marchitez del melón (Cucumis melo L.) mediante RAPD, comparándose tales resultados con la identificación taxonómica en base a caracteres morfológicos y patogénicos. Se estudiaron 18 aislamientos colectados de cultivos comerciales de melón establecidos entre la III y IV Región, Chile. Las observaciones morfológicas determinaron que once aislamientos correspondían a F. oxysporum, cuatro a F. semitectum, uno a F. solani y uno a F. subglutinans. Aislamientos identificados como F. oxysporum produjeron síntomas de marchitez solamente en plantas de melón, identificándolos, por lo tanto, como F. oxysporum f. sp. melonis (Fom). Los perfiles de ADN obtenidos mediante RAPD así como los análisis de... |
| Tipo: Journal article |
Palavras-chave: RAPD; Diversidad genética. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072001000300004 |
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| GOMEZ,PATRICIA I; GONZALEZ,MARIELA A. |
| Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR). Twenty of the 50 random primers (D, P, OPA and OPD series) that were tested amplified reproducible bands and were useful for comparative analysis of the strains. Of 107 polymorphic genetic markers, 49 were strain-specific. A great genetic variability was found among the strains in spite of their geographic proximity. In addition, phenetic analysis of the data showed close agreement between the morpho-physiological attributes and the genetic diversity of the strains |
| Tipo: Journal article |
Palavras-chave: SS-carotene; Dunaliella salina; Genetic polymorphism; RAPD. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100012 |
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| Freire,Laurineide Lopes de Carvalho; Costa,Ana Bolena Lima da; Góes,Larissa Brandão; Oliveira,Neiva Tinti de. |
| Five mutants (MaE10, MaE27, MaE24, MaE41 e MaE49) of Metarhizium anisopliae wild strain E9 were analysed for DNA profile through the RAPD technique and for changes in total protein content by spectrophotometry, polyacrylamide gel electrophoresis and densitometry. The pattern of RAPD markers showed genetic polymorphism among the strains: out of twenty primers seven were selected, producing 113 bands. Forty seven bands were present in all strains (41.6% of monomorphic bands) and 66 showed polymorphism (58.4%). The mean coefficient of similarity among all strains was 0.75 (75%). The total protein content varied, staining in the interval of 6.0-8.0 µg/µl. The electrophoresis analysis, through zymogram and protein fraction profiles by densitometry, allowed the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metarhizium anisopliae; Mutants; RAPD; Total protein. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200004 |
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| Maki,Cristina Sayuri; Teixeira,Flavia França; Paiva,Edilson; Paccola-Meirelles,Luzia Doretto. |
| The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37ºC) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28ºC. The water content was lower in strains grown at 37ºC. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Edible mushroom; RAPD; Mycelial growth. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300002 |
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| Glienke-Blanco,Chirlei; Aguilar-Vildoso,Carlos Ivan; Vieira,Maria Lúcia Carneiro; Barroso,Paulo Augusto Vianna; Azevedo,João Lúcio. |
| During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Endophytes; Genetic variability; Guignardia citricarpa; Mandarin; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200021 |
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| Martins Filho,Sebastião; Sediyama,Carlos Sigueyuki; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de. |
| The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; RAPD; SCAR; Soybean; Cercospora sojina; Frogeye leaf spot. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012 |
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| Dergam,Jorge A.; Paiva,Samuel R.; Schaeffer,Carlos E.; Godinho,Alexandre L.; Vieira,Fabio. |
| In the Rio Doce basin of southeastern Brazil, the freshwater fish Hoplias malabaricus (trahira) is a widespread predatory characin and one of the few resilient native fishes in a highly impacted lake system. In order to test for genetic differentiation in populations within this basin and for biogeographic relationships among populations of this species in other basins, a study was conducted using RAPD-PCR analysis of Rio Doce samples (N = 63) and phylogeographic analyses with mitochondrial DNA (mtDNA) haplotypes, including the Rio Grande and Macacu river basins. In the Rio Doce basin, the patterns of genetic similarity of RAPD-PCR markers (individual fingerprinting and Neis genetic distance) suggest the existence of two genetically different groups, one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; SSCP; MtDNA; Phylogeography; Hoplias malabaricus; Conservation genetics. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400005 |
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| Registros recuperados: 233 | |
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